7RM9
Human Malate Dehydrogenase I (MDHI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-15 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.503, 84.312, 77.652 |
| Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
| Resolution | 38.820 - 1.650 |
| R-factor | 0.1674 |
| Rwork | 0.166 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mdh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.910 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.634 | 1.678 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.048 | 0.321 |
| Rmeas | 0.061 | 0.385 |
| Rpim | 0.032 | 0.209 |
| Number of reflections | 73795 | 3944 |
| <I/σ(I)> | 13.8 | 3.2 |
| Completeness [%] | 92.6 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.998 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 293 | 28% PEG 4000, 100 mM Malonate pH 7.25, 0.15 mM ammonium acetate |
