7RLW

Antibody 2F2 in complex with P. vivax CSP peptide GDRAAGQPAGDRAAGQPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-02-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033167
Spacegroup name	C 1 2 1
Unit cell lengths	92.911, 60.396, 158.300
Unit cell angles	90.00, 101.46, 90.00

Refinement procedure

Resolution	29.210 - 2.540
R-factor	0.2038
Rwork	0.201
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xiw
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.210	2.650
High resolution limit [Å]	2.540	2.540
Number of reflections	28642	3484
<I/σ(I)>	6.7	1.5
Completeness [%]	99.8	100
Redundancy	6.3	6.1
CC(1/2)	0.994	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M CHES pH 9.6 and 20% (w/v) PEG 8000