7RLK
Wallaby TTR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9984 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.516, 112.152, 245.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.240 - 2.690 |
| R-factor | 0.1891 |
| Rwork | 0.188 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u2i |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.759 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.870 | 2.710 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.073 | 0.585 |
| Number of reflections | 28992 | 3451 |
| <I/σ(I)> | 31.3 | |
| Completeness [%] | 99.8 | |
| Redundancy | 14.2 | |
| CC(1/2) | 0.909 | 0.952 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 25 mM Tris, 150 mM NaCl pH 8.0 |
