7RL3
Structure of Drosophila melanogaster Plk4 PB3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-08-07 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.711, 53.095, 41.637 |
| Unit cell angles | 90.00, 107.92, 90.00 |
Refinement procedure
| Resolution | 39.617 - 1.750 |
| R-factor | 0.1964 |
| Rwork | 0.192 |
| R-free | 0.23270 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.644 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (v1.8) |
| Refinement software | PHENIX (v1.8) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.048 | 0.035 | 0.251 |
| Number of reflections | 14468 | 1585 | 961 |
| <I/σ(I)> | 20.2 | ||
| Completeness [%] | 93.4 | 99.6 | 62.4 |
| Redundancy | 3.5 | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | D.m. Plk4 PB3 was crystallized using a mother liquor (1 ml) containing 32% PEG 4000, 200 mM Li2SO4, and 200 mM Tris at pH 8.5. The hanging drop initial condition was 2 ul mother liquor plus 2 ul (15 mg/ml stock) Plk4 PB3. Crystals were transferred to a cryo condition containing mother liquor plus 20% ethylene glycol |
