7RJK

Crystal structure of human Bromodomain containing protein 3 (BRD3) in complex with hnRNPK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.92
Spacegroup name	P 41 21 2
Unit cell lengths	49.658, 49.658, 255.611
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.830 - 1.850
R-factor	0.1751
Rwork	0.173
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qju
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.830	24.830	1.890
High resolution limit [Å]	1.850	9.060	1.850
Rmerge	0.123	0.060	0.567
Rmeas	0.125	0.061	0.580
Rpim	0.025	0.013	0.122
Total number of observations	735477	6326	38967
Number of reflections	28702	320	1684
<I/σ(I)>	20.9	32.5	6.6
Completeness [%]	100.0	96.3	100
Redundancy	25.6	19.8	23.1
CC(1/2)	0.999	0.999	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	292	2.0 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate, 0.2 M potassium/sodium tartrate tetrahydrate