7RIY

RNA polymerase II elongation complex with hairpin polyamide Py-Im 1, scaffold 2 soaked with UTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	167.775, 222.760, 192.956
Unit cell angles	90.00, 100.54, 90.00

Refinement procedure

Resolution	49.533 - 3.700
R-factor	0.2331
Rwork	0.232
R-free	0.28060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rim
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.533	49.530	3.700
High resolution limit [Å]	3.630	18.510	3.630
Rmerge	0.521	0.075	3.075
Rmeas	0.559	0.082	3.297
Rpim	0.202	0.032	1.186
Total number of observations		3683	34030
Number of reflections	78506	585	4429
<I/σ(I)>	4.5	19.9	0.9
Completeness [%]	99.9	94	100
Redundancy	7.6	6.3	7.7
CC(1/2)	0.979	0.995	0.288

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000