7RIW

RNA polymerase II elongation complex scaffold 2, without polyamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	165.012, 222.435, 192.301
Unit cell angles	90.00, 99.61, 90.00

Refinement procedure

Resolution	47.805 - 3.200
R-factor	0.2284
Rwork	0.228
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rim
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.950	48.950	3.220
High resolution limit [Å]	3.170	17.360	3.170
Rmerge	0.410
Rmeas	0.439
Rpim	0.158
Number of reflections	115583	690	5744
<I/σ(I)>	5.3
Completeness [%]	100.0
Redundancy	7.7
CC(1/2)	0.986	0.997	0.229

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000