7RIB
Griffithsin mutant Y28F/Y68F/Y110F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2018-05-01 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.201, 47.792, 95.213 |
| Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
| Resolution | 24.980 - 2.100 |
| R-factor | 0.1777 |
| Rwork | 0.172 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2guc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.566 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.17.1) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.200 | 0.095 |
| Number of reflections | 20011 | 964 |
| <I/σ(I)> | 4.8 | 1.3 |
| Completeness [%] | 96.4 | 94.4 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M bis-tris buffer, pH 6.5, 1.0 M ammonium sulfate |
