7RHC

A new fluorescent protein darkmRuby at pH 9.0

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1158
Spacegroup name	C 1 2 1
Unit cell lengths	107.540, 76.930, 66.650
Unit cell angles	90.00, 90.99, 90.00

Refinement procedure

Resolution	45.890 - 2.800
R-factor	0.2004
Rwork	0.197
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u0m
RMSD bond length	0.008
RMSD bond angle	1.583
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.890	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	13453	1955
<I/σ(I)>	9.1
Completeness [%]	99.4
Redundancy	4.71
CC(1/2)	0.994	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.1 M CHES: NaOH pH 9.5, 30% (w/v) PEG3000