7RHA

A new fluorescent protein darkmRuby at pH 5.0

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	73.571, 101.685, 134.983
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.580 - 1.800
R-factor	0.1773
Rwork	0.176
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u0l
RMSD bond length	0.009
RMSD bond angle	1.569
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.620	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	47367	6812
<I/σ(I)>	10.4
Completeness [%]	100.0	99.9
Redundancy	7.36
CC(1/2)	0.997	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.2 M lithium sulfate, 0.1 M sodium acetate: acetic acid pH 4.5, 30% (w/v) PEG8000