7RGT
The crystal structure of RocC, containing FinO domain, 1-126
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.010, 100.303, 110.718 |
| Unit cell angles | 90.00, 107.57, 90.00 |
Refinement procedure
| Resolution | 45.300 - 2.020 |
| R-factor | 0.1938 |
| Rwork | 0.193 |
| R-free | 0.21260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RocC(24-126) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.708 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Number of reflections | 94465 | 4477 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 99.7 | 95.6 |
| Redundancy | 6.2 | 4.7 |
| CC(1/2) | 0.989 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 289.15 | 0.2M Ammonium Sulfate, 0.1M HEPES:NaOH, pH 7.3, 20% PEG 3350 with various additives |
