7RGS

The crystal structure of RocC, containing FinO domain, 24-126

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-24
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	55.559, 81.772, 49.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.420 - 2.100
R-factor	0.1642
Rwork	0.161
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dvo 3mw6
RMSD bond length	0.006
RMSD bond angle	0.740
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	13499	619
<I/σ(I)>	35.7
Completeness [%]	97.9	91.8
Redundancy	36.5	28.7
CC(1/2)	1.000	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289.15	0.2M Ammonium Acetate, 0.1M HEPES pH 7.5, 25% PEG 3350