7RG3

Importin alpha2 in complex with MERS ORF4B R37A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-12-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.731, 89.960, 100.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.620 - 2.000
R-factor	0.1775
Rwork	0.177
R-free	0.19420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bvt
RMSD bond length	0.003
RMSD bond angle	0.558
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.990	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.064	0.600
Rpim	0.038	0.350
Number of reflections	48968	3566
<I/σ(I)>	15.4	3.1
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.7 M Sodium Citrate, 0.1 M HEPES, pH 7.5