7RG1

Importin alpha2 in complex with MERS ORF4B H26A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-12-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.951, 89.650, 100.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.630 - 1.850
R-factor	0.2009
Rwork	0.200
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bvt
RMSD bond length	0.002
RMSD bond angle	0.517
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER (1.19rc4_4035)
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.880	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.098	1.340
Rpim		0.710
Number of reflections	61555	3637
<I/σ(I)>	14.3
Completeness [%]	99.8	96.9
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.7 M Sodium Citrate, 0.1 M HEPES, pH 7.5