7RFX

Importin alpha2 in complex with MERS ORF4B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	CCD
Collection date	2020-08-06
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	79.312, 89.680, 99.361
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.930 - 2.100
R-factor	0.1976
Rwork	0.197
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bvt
RMSD bond length	0.002
RMSD bond angle	0.525
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.930	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.160
Number of reflections	42012	3409
<I/σ(I)>	4.9
Completeness [%]	99.8
Redundancy	4.9
CC(1/2)	0.990	0.562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.7M sodium citrate (pH 7.0) and 10 mM DTT