7RFV
Tailspike protein 4 (TSP4) from phage CBA120, residues 1-250, obtained in the presence of PEG8000
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 84.683, 84.683, 225.073 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.450 - 3.200 |
| R-factor | 0.2049 |
| Rwork | 0.201 |
| R-free | 0.30080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7rej |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.484 |
| Data reduction software | TRUNCATE |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.640 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | |
| Number of reflections | 7883 | 3737 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.0 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.999 | 0.077 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 10.5 | 295 | 20% w/v PEG8000, 0.2 M sodium chloride, 0.1 M CHAPS, pH 10.5 |
