7RFO

SeMet Tailspike protein 4 (TSP4) phage CBA120, residues 1-335, obtained in the presence of LiSO4

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	P 41 21 2
Unit cell lengths	67.900, 67.900, 607.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.020
R-factor	0.2281
Rwork	0.225
R-free	0.29560
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.843
Data reduction software	TRUNCATE
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.010	3.200
High resolution limit [Å]	3.020	3.020
Rmerge	0.228
Number of reflections	28173	3193
<I/σ(I)>	20.4
Completeness [%]	94.4
Redundancy	66.9
CC(1/2)	0.999	0.256

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	1.6 M lithium sulfate, 0.1 M sodium chloride, 0.1 M HEPES, pH 7.5