7REZ

MYCOBACTERIUM ABSCESSUS TRNA METHYLTRANSFERASE IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	74.242, 79.094, 86.631
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.210 - 1.640
R-factor	0.188
Rwork	0.186
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nvr
RMSD bond length	0.006
RMSD bond angle	0.974
Data reduction software	ADDREF
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.220	1.681
High resolution limit [Å]	1.640	1.640
Number of reflections	63130	4406
<I/σ(I)>	0.699
Completeness [%]	99.6	95.5
Redundancy	13.1
CC(1/2)	1.000	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Protein at 24mg/ml was incubated with 10mM SAH in DMSO in a 1 to 1 ratio; Buffer 2 M ammonium sulfate, 0.1 M sodium cacodylate pH 6.5. Drops were formed 1 to 1 ratio and crystals cryo protected by adding 1 microliter of 50 percent Glycerol