7REY
MYCOBACTERIUM ABSCESSUS TRNA METHYLTRANSFERASE IN APO FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.785, 79.375, 86.604 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.260 - 1.870 |
| R-factor | 0.17688 |
| Rwork | 0.174 |
| R-free | 0.22625 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6nvr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.576 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.260 | 1.910 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.062 | |
| Number of reflections | 42678 | 2711 |
| <I/σ(I)> | 16 | 1.1 |
| Completeness [%] | 99.0 | 86.7 |
| Redundancy | 6.6 | 5.8 |
| CC(1/2) | 0.999 | 0.612 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2ul protein at concentration 24mg/ml, and 2ul buffer (0.1 M sodium cacodylate, 2 M ammonium sulfate pH 6.5), 1uL 50% Glycerol |
