7REW
Crystal Structure of IL-13 in complex with MMAb3 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-04 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.790, 74.352, 76.530 |
| Unit cell angles | 96.85, 109.89, 112.49 |
Refinement procedure
| Resolution | 35.960 - 2.100 |
| R-factor | 0.2473 |
| Rwork | 0.245 |
| R-free | 0.29090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cqi |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.679 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.3) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 68.940 | 68.940 | 2.170 |
| High resolution limit [Å] | 2.100 | 8.930 | 2.100 |
| Rmerge | 0.051 | 0.048 | 0.381 |
| Rmeas | 0.068 | 0.065 | 0.519 |
| Rpim | 0.044 | 0.043 | 0.351 |
| Total number of observations | 115864 | 1661 | 9760 |
| Number of reflections | 54189 | 785 | 4598 |
| <I/σ(I)> | 7.2 | 11.8 | 2.8 |
| Completeness [%] | 88.0 | 96.3 | 90.4 |
| Redundancy | 2.1 | 2.1 | 2.1 |
| CC(1/2) | 0.993 | 0.976 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293 | 0.2 M LiSO4, 0.1 M Tris HCl pH 8.5, 25% PEG 5,000 MME |
