7RE4

Apo Hemophilin from A. baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	105
Detector technology	CCD
Collection date	2016-07-19
Detector	RAYONIX MX300HE
Wavelength(s)	0.9789
Spacegroup name	C 2 2 21
Unit cell lengths	71.110, 145.560, 153.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.870 - 1.870
R-factor	0.1739
Rwork	0.172
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7red
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.870	1.937
High resolution limit [Å]	1.870	1.870
Number of reflections	65838	6505
<I/σ(I)>	9.93
Completeness [%]	100.0
Redundancy	15
CC(1/2)	0.995	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	100 mM Hepes pH 7, 10% (w/v) PEG 6K