7RDN

Crystal structure of S. cerevisiae pre-mRNA leakage protein 39 (Pml39)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.282
Spacegroup name	P 31 2 1
Unit cell lengths	53.010, 53.010, 171.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.340 - 2.490
R-factor	0.2341
Rwork	0.233
R-free	0.26250
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.491
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.340	2.580
High resolution limit [Å]	2.490	2.490
Rmerge	0.045	0.889
Number of reflections	18812	1837
<I/σ(I)>	30.4	3.16
Completeness [%]	99.6	99.67
Redundancy	11.3	11.4
CC(1/2)	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	298	PEG 8000, HEPES-HCl pH 7.7