7RDM
Crystal structure of PCDN-38B, a broadly neutralizing anti-HIV antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03309 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.696, 68.065, 77.937 |
| Unit cell angles | 90.00, 96.65, 90.00 |
Refinement procedure
| Resolution | 45.500 - 2.080 |
| R-factor | 0.1923 |
| Rwork | 0.191 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bzd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.578 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 36353 | 5694 |
| <I/σ(I)> | 9 | 1.2 |
| Completeness [%] | 99.1 | 96.8 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 277.15 | 1.6 M sodium dihydrogen phosphate, 0.4 M dipotassium hydrogen phosphate, 0.1 M phosphate-citrate |
