7RDG

Crystal structure of D103A human Galectin-7 mutant in presence of lactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.5214
Spacegroup name	P 1 21 1
Unit cell lengths	30.330, 112.280, 77.040
Unit cell angles	90.00, 91.08, 90.00

Refinement procedure

Resolution	38.510 - 3.000
R-factor	0.2096
Rwork	0.204
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bkz
RMSD bond length	0.015
RMSD bond angle	1.699
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.510	36.430	3.110
High resolution limit [Å]	3.000	6.450	3.000
Rmerge	0.072	0.053	0.158
Rmeas	0.086	0.063	0.191
Rpim	0.047	0.035	0.105
Number of reflections	10243	1053	1023
<I/σ(I)>	12.8	16.9	7.3
Completeness [%]	100.0	99.3	98.2
Redundancy	3.2	3.3	3.2
CC(1/2)	0.993	0.995	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	295.15	0.1 M Tris pH 7.3, 0.1 M NaCl, 18% P400, 26% PEG 3350