7RDG
Crystal structure of D103A human Galectin-7 mutant in presence of lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.5214 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.330, 112.280, 77.040 |
| Unit cell angles | 90.00, 91.08, 90.00 |
Refinement procedure
| Resolution | 38.510 - 3.000 |
| R-factor | 0.2096 |
| Rwork | 0.204 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bkz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.699 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.510 | 36.430 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.450 | 3.000 |
| Rmerge | 0.072 | 0.053 | 0.158 |
| Rmeas | 0.086 | 0.063 | 0.191 |
| Rpim | 0.047 | 0.035 | 0.105 |
| Number of reflections | 10243 | 1053 | 1023 |
| <I/σ(I)> | 12.8 | 16.9 | 7.3 |
| Completeness [%] | 100.0 | 99.3 | 98.2 |
| Redundancy | 3.2 | 3.3 | 3.2 |
| CC(1/2) | 0.993 | 0.995 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 295.15 | 0.1 M Tris pH 7.3, 0.1 M NaCl, 18% P400, 26% PEG 3350 |
