7RDE
Human Triose Phosphate Isomerase Q181P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.189, 77.822, 84.295 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.330 - 1.310 |
| R-factor | 0.1373 |
| Rwork | 0.137 |
| R-free | 0.16200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6nlh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.180 | 1.331 |
| High resolution limit [Å] | 1.308 | 1.308 |
| Number of reflections | 115131 | 5679 |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 98.8 | 98.5 |
| Redundancy | 6.6 | 6.3 |
| CC(1/2) | 0.998 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 30% PEG 3350, 50mM Potassium Bromide, Tris pH7.5 |
