7RDB

Crystal structure of Tspan15 large extracellular loop (Tspan15 LEL)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	83.570, 91.950, 112.380
Unit cell angles	87.55, 86.52, 86.24

Refinement procedure

Resolution	48.670 - 2.520
R-factor	0.2356
Rwork	0.235
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rd5
RMSD bond length	0.012
RMSD bond angle	1.406
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.670	2.610
High resolution limit [Å]	2.520	2.520
Rmerge	0.146	1.290
Rmeas	0.160	1.451
Rpim	0.065	0.645
Number of reflections	111846	11044
<I/σ(I)>	6.85	1.34
Completeness [%]	99.4	97.51
Redundancy	5.7	4.7
CC(1/2)	0.996	0.441

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	293	0.1 M MES pH 6.9, 34-35 % polyethylene p-400