7RCQ
Crystal structure of triosephosphate isomerase from Ktedonobacter racemifer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-07-28 |
| Detector | AGILENT TITAN CCD |
| Wavelength(s) | 1.5406 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.580, 54.741, 74.843 |
| Unit cell angles | 90.00, 115.20, 90.00 |
Refinement procedure
| Resolution | 13.180 - 1.700 |
| R-factor | 0.1594 |
| Rwork | 0.158 |
| R-free | 0.18920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y8f |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.847 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.180 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 25558 | 1282 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 98.2 | |
| Redundancy | 3 | |
| CC(1/2) | 0.998 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M ammonium nitrate, 20 % w/v PEG 3350 |
