7RBO
Human DNA polymerase beta crosslinked complex, 60 min Ca to Mg exchange
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.416, 81.772, 55.736 |
| Unit cell angles | 90.00, 109.08, 90.00 |
Refinement procedure
| Resolution | 45.270 - 2.960 |
| R-factor | 0.2519 |
| Rwork | 0.248 |
| R-free | 0.28370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rq1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.097 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.270 | 45.270 | 3.140 |
| High resolution limit [Å] | 2.960 | 8.890 | 2.960 |
| Rmerge | 0.079 | 0.040 | 0.411 |
| Rmeas | 0.093 | 0.047 | 0.485 |
| Rpim | 0.050 | 0.025 | 0.255 |
| Total number of observations | 33098 | 1308 | 5400 |
| Number of reflections | 9589 | 373 | 1533 |
| <I/σ(I)> | 11.9 | 30 | 3.1 |
| Completeness [%] | 97.4 | 97.3 | 97.2 |
| Redundancy | 3.5 | 3.5 | 3.5 |
| CC(1/2) | 0.996 | 0.996 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Citrate pH 5.5, 18% PEG6000 |
