7RBK
Human DNA polymerase beta crosslinked complex, 40 s Ca to Mg exchange
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.558, 80.398, 55.419 |
| Unit cell angles | 90.00, 107.64, 90.00 |
Refinement procedure
| Resolution | 48.180 - 2.200 |
| R-factor | 0.2004 |
| Rwork | 0.198 |
| R-free | 0.25130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rpy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.180 | 48.180 | 2.260 |
| High resolution limit [Å] | 2.200 | 9.050 | 2.200 |
| Rmerge | 0.085 | 0.056 | 0.493 |
| Rmeas | 0.092 | 0.061 | 0.537 |
| Rpim | 0.035 | 0.023 | 0.210 |
| Total number of observations | 144142 | 2077 | 11917 |
| Number of reflections | 21335 | 324 | 1849 |
| <I/σ(I)> | 15.4 | 33 | 4 |
| Completeness [%] | 98.5 | 98.2 | 99.1 |
| Redundancy | 6.8 | 6.4 | 6.4 |
| CC(1/2) | 0.998 | 0.997 | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Citrate pH 5.5, 18% PEG6000 |
