7RBI
Human DNA polymerase beta crosslinked complex, 20 s Ca to Mg exchange
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.019, 79.297, 55.125 |
| Unit cell angles | 90.00, 105.86, 90.00 |
Refinement procedure
| Resolution | 44.120 - 1.930 |
| R-factor | 0.1875 |
| Rwork | 0.186 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rpy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.579 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.150 | 47.150 | 1.970 |
| High resolution limit [Å] | 1.930 | 9.050 | 1.930 |
| Rmerge | 0.034 | 0.015 | 0.340 |
| Rmeas | 0.040 | 0.018 | 0.406 |
| Rpim | 0.021 | 0.009 | 0.218 |
| Total number of observations | 102846 | 1055 | 6556 |
| Number of reflections | 29463 | 300 | 1999 |
| <I/σ(I)> | 14.7 | 42.3 | 2.4 |
| Completeness [%] | 96.3 | 96.2 | 95.7 |
| Redundancy | 3.5 | 3.5 | 3.3 |
| CC(1/2) | 0.999 | 0.999 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Citrate pH 5.5, 18% PEG6000 |
