7RBG
Human DNA polymerase beta crosslinked ternary complex 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-01 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.510, 78.430, 57.190 |
| Unit cell angles | 90.00, 113.22, 90.00 |
Refinement procedure
| Resolution | 51.070 - 1.900 |
| R-factor | 0.1811 |
| Rwork | 0.179 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kld |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.483 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 52.560 | 52.560 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.074 | 0.027 | 0.385 |
| Rmeas | 0.087 | 0.033 | 0.459 |
| Rpim | 0.045 | 0.019 | 0.247 |
| Total number of observations | 112661 | 944 | 6250 |
| Number of reflections | 32605 | 299 | 2028 |
| <I/σ(I)> | 12 | 28 | 3.2 |
| Completeness [%] | 91.5 | 86.4 | 88.3 |
| Redundancy | 3.5 | 3.2 | 3.1 |
| CC(1/2) | 0.996 | 0.994 | 0.581 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Citrate pH 5.5, 18% PEG6000 |
