7RBF
Human DNA polymerase beta crosslinked binary complex - B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.680, 79.571, 54.828 |
| Unit cell angles | 90.00, 91.68, 90.00 |
Refinement procedure
| Resolution | 45.130 - 1.840 |
| R-factor | 0.1776 |
| Rwork | 0.176 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bpx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.725 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.130 | 45.130 | 1.880 |
| High resolution limit [Å] | 1.840 | 9.030 | 1.840 |
| Rmerge | 0.048 | 0.020 | 0.401 |
| Rmeas | 0.056 | 0.024 | 0.475 |
| Rpim | 0.029 | 0.014 | 0.250 |
| Total number of observations | 151851 | 758 | 8516 |
| Number of reflections | 40114 | 262 | 2461 |
| <I/σ(I)> | 12.6 | 26 | 2.2 |
| Completeness [%] | 98.9 | 71.3 | 97.8 |
| Redundancy | 3.8 | 2.9 | 3.5 |
| CC(1/2) | 0.999 | 0.999 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M Citrate pH 5.5, 18% PEG6000 |
