7RAR
Structure of Q67H mutant of disulfide stabilized HIV-1 CA hexamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2020-10-13 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 6 |
Unit cell lengths | 91.849, 91.849, 57.775 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.750 - 2.150 |
R-factor | 0.2096 |
Rwork | 0.208 |
R-free | 0.23980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h47 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.717 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.750 | 2.220 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.182 | 1.590 |
Number of reflections | 15259 | 1325 |
<I/σ(I)> | 12.2 | 1.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.8 | 10.2 |
CC(1/2) | 0.997 | 0.667 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.425M NaI, 4% peg 3350, 6% glycerol, 0.1M sodium cacodylate trihydrate pH 6.5 |