7RAB
Crystal structure of a dodecameric multicopper oxidase from M. hydrothermalis in a cubic lattice
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.37623 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 224.241, 224.241, 224.241 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.060 - 1.920 |
| R-factor | 0.2715 |
| Rwork | 0.270 |
| R-free | 0.29830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3GDC_A |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.894 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 129.470 | 129.470 | 2.020 |
| High resolution limit [Å] | 1.915 | 6.060 | 1.920 |
| Rmerge | 0.170 | 0.066 | 1.604 |
| Rmeas | 0.173 | 0.067 | 1.625 |
| Rpim | 0.028 | 0.011 | 0.256 |
| Total number of observations | 362861 | 1665919 | |
| Number of reflections | 284806 | 9410 | 41439 |
| <I/σ(I)> | 10.6 | 27.4 | 2.5 |
| Completeness [%] | 99.7 | 99.8 | 99.8 |
| Redundancy | 39 | 38.6 | 40.2 |
| CC(1/2) | 1.000 | 1.000 | 0.962 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium sulfate, 0.1 M HEPES 7.5, 25% PEG 335 |
