7R9Z

LC3A in complex with Fragment 2-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	I 41
Unit cell lengths	93.463, 93.463, 32.322
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.730 - 1.720
R-factor	0.195
Rwork	0.192
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wal
RMSD bond length	0.008
RMSD bond angle	0.978
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.730	1.750
High resolution limit [Å]	1.720	1.720
Rmerge	0.052	1.250
Number of reflections	15116	1508
<I/σ(I)>	2
Completeness [%]	100.0
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Sodium formate, 20% PEG 3350