7R98

Structure of the SARS-CoV-2 N protein RNA-binding domain bound to single-domain antibody B6

Replaces:  7N0S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	99.613, 175.061, 111.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.530 - 2.510
R-factor	0.2285
Rwork	0.226
R-free	0.27320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cdz
RMSD bond length	0.016
RMSD bond angle	1.493
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.000	2.610
High resolution limit [Å]	2.510	2.510
Rmerge	0.214
Rpim	0.060	1.161
Number of reflections	33832	3761
<I/σ(I)>	9.7	0.7
Completeness [%]	100.0	100
Redundancy	13.7
CC(1/2)	0.996	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium citrate pH 5.6, 0.1 M sodium-potassium tartrate, and 19% PEG 3350