7R97

Crystal structure of postcleavge complex of Escherichia coli RNase III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-15
Detector	RAYONIX MX300-HS
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	56.929, 65.753, 84.519
Unit cell angles	90.00, 102.09, 90.00

Refinement procedure

Resolution	28.308 - 1.804
R-factor	0.1716
Rwork	0.171
R-free	0.20210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nug
RMSD bond length	0.008
RMSD bond angle	1.018
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.121	0.065	1.151
Rmeas	0.132	0.072	1.261
Rpim	0.053	0.030	0.504
Total number of observations	362726
Number of reflections	55901	5697	5494
<I/σ(I)>	10.7
Completeness [%]	99.5	99.1	98.4
Redundancy	6.5	5.9	6.1
CC(1/2)		0.993	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	292	PEG 3350, KBr, etc.