7R97
Crystal structure of postcleavge complex of Escherichia coli RNase III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-15 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.929, 65.753, 84.519 |
| Unit cell angles | 90.00, 102.09, 90.00 |
Refinement procedure
| Resolution | 28.308 - 1.804 |
| R-factor | 0.1716 |
| Rwork | 0.171 |
| R-free | 0.20210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nug |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.018 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.121 | 0.065 | 1.151 |
| Rmeas | 0.132 | 0.072 | 1.261 |
| Rpim | 0.053 | 0.030 | 0.504 |
| Total number of observations | 362726 | ||
| Number of reflections | 55901 | 5697 | 5494 |
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 99.5 | 99.1 | 98.4 |
| Redundancy | 6.5 | 5.9 | 6.1 |
| CC(1/2) | 0.993 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 292 | PEG 3350, KBr, etc. |
