7R8X

Crystal Structure of the LNK SH2 Domain in Complex with an EPOR pY454 Phosphopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953740
Spacegroup name	P 61
Unit cell lengths	86.157, 86.157, 38.052
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.900 - 2.300
R-factor	0.1998
Rwork	0.197
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hdv
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.900	2.383
High resolution limit [Å]	2.300	2.301
Rmerge	0.044	0.492
Rmeas	0.047	0.539
Rpim	0.015
Number of reflections	7318	724
<I/σ(I)>	31	2.81
Completeness [%]	99.8	99.45
Redundancy	9.6
CC(1/2)	1.000	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	277.15	20% PEG8000, 0.05 M magnesium acetate, 0.1 M Tris, ph 8.5