7R8G
Crystal structure of Pseudooceanicola lipolyticus Argonaute bound to 5' OH guide DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 3 |
Unit cell lengths | 200.598, 200.598, 200.598 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.860 - 2.500 |
R-factor | 0.2128 |
Rwork | 0.211 |
R-free | 0.25410 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.083 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.035 | 0.944 |
Number of reflections | 46475 | 2292 |
<I/σ(I)> | 27.3 | 1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.1 | 10.3 |
CC(1/2) | 0.999 | 0.344 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 100 mM Tris-HCl pH 8.0, 8% PEG 8000 |