7R8F

Crystal structure of Pseudooceanicola lipolyticus Argonaute

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9687
Spacegroup name	I 2 3
Unit cell lengths	197.938, 197.938, 197.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.480 - 3.160
R-factor	0.2185
Rwork	0.214
R-free	0.25810
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.694
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Rpim	0.070
Number of reflections	23413	2310
<I/σ(I)>	24
Completeness [%]	100.0	100
Redundancy	18.2
CC(1/2)		0.331

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	100 mM Tris-HCl pH 8.0, 8% PEG 8000