7R85

Structure of mouse Bai1 (ADGRB1) TSR3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 42 2 2
Unit cell lengths	43.915, 43.915, 64.312
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.915 - 1.450
R-factor	0.1817
Rwork	0.178
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	complex structure of BAI1-RTN4R
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.920	43.920	1.470
High resolution limit [Å]	1.450	7.940	1.450
Rmerge	0.060	0.043	3.881
Rmeas	0.063	0.046	4.075
Rpim	0.019	0.016	1.221
Total number of observations	142182	917	6194
Number of reflections	11734	102	570
<I/σ(I)>	15	37.2	0.6
Completeness [%]	99.9	99.4	99.4
Redundancy	12.1	9	10.9
CC(1/2)	0.996	0.999	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1 M succinic acid, 0.1 M HEPES, pH 7.0 and 1% PEG 2000 MME