7R74
Crystal structure of llama VHH antibody in complex with HIV-1 HXBC2 gp120 core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-10-31 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.077, 87.403, 91.581 |
| Unit cell angles | 90.00, 109.12, 90.00 |
Refinement procedure
| Resolution | 49.560 - 2.760 |
| R-factor | 0.2145 |
| Rwork | 0.212 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TMF |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19-4092) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 7.460 | 2.750 |
| Rmerge | 0.139 | 0.112 | 0.368 |
| Total number of observations | 69593 | ||
| Number of reflections | 21712 | 1201 | 572 |
| <I/σ(I)> | 8.6 | ||
| Completeness [%] | 94.0 | 100 | 50.2 |
| Redundancy | 3.2 | 3.5 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.0 M Li2SO4 and 100 mM HEPES pH 7.5 |
