7R6S

Crystal Structure of the Putative Bacteriophage Protein from Stenotrophomonas maltophilia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97848
Spacegroup name	P 1 21 1
Unit cell lengths	52.436, 90.670, 72.067
Unit cell angles	90.00, 103.99, 90.00

Refinement procedure

Resolution	28.710 - 1.900
R-factor	0.1905
Rwork	0.188
R-free	0.23080
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.269
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.082	0.928
Rmeas	0.090	1.022
Rpim	0.036	0.423
Number of reflections	51491	2592
<I/σ(I)>	20.1	2
Completeness [%]	98.9	99.6
Redundancy	5.9	5.6
CC(1/2)	0.999	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	292	Protein: 8.4mg/ml, 0.15M Sodium chloride, 0.01M Tris pH 8.3; Screen: PEGs II (F6), 0.2M Ammonium sulfate, 0.1M tri-Sodium citrate pH 5.6, 25% (w/v) PEG 4000.