7R5A

Vibrio cholera ParD2:ParE2 antitoxin:toxin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978565
Spacegroup name	P 43 21 2
Unit cell lengths	65.780, 65.780, 127.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.510 - 2.950
R-factor	0.2676
Rwork	0.263
R-free	0.30810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kxe
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.510	3.056
High resolution limit [Å]	2.950	2.950
Rmerge	0.163
Number of reflections	78228	7628
<I/σ(I)>	10.62
Completeness [%]	99.8
Redundancy	12.3
CC(1/2)	0.999	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Potassium chloride, 0.1 M Sodium HEPES, pH 7, 15 % PEG 5000 MME