7R57
Escherichia coli type II Asparaginase N24S mutant in its apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07252 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.139, 62.440, 143.409 |
| Unit cell angles | 90.00, 118.19, 90.00 |
Refinement procedure
| Resolution | 47.090 - 1.400 |
| R-factor | 0.1652 |
| Rwork | 0.165 |
| R-free | 0.18450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eca |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.983 |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.200 | 48.200 | 1.390 |
| High resolution limit [Å] | 1.340 | 5.190 | 1.340 |
| Rmerge | 0.047 | 0.031 | 1.023 |
| Rmeas | 0.058 | 0.038 | 1.263 |
| Rpim | 0.033 | 0.022 | 0.731 |
| Total number of observations | 724964 | 13044 | 67722 |
| Number of reflections | 261282 | 4627 | 25496 |
| <I/σ(I)> | 10.1 | 32.8 | 0.9 |
| Completeness [%] | 98.5 | 97.3 | 98.1 |
| Redundancy | 2.8 | 2.8 | 2.7 |
| CC(1/2) | 0.998 | 0.997 | 0.388 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) 100 mM pH 8.0, PEG 8000 5%, ETHYLENE GLYCOL 4% |
