7R4V
Strep-tag FtrA-P19 from Rubrivivax gelatinosus in complex with an endogenous CU1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.379288 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.400, 65.890, 70.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.020 - 1.300 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7r3p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.020 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.076 | 0.542 |
| Number of reflections | 114499 | 1375 |
| <I/σ(I)> | 14.99 | |
| Completeness [%] | 84.7 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.998 | 0.654 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 20% PEG 1500, 0.1 M HEPES |
