7R4B
The Bacillus pumilus chorismate mutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-02 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.898, 94.864, 77.305 |
| Unit cell angles | 90.00, 107.49, 90.00 |
Refinement procedure
| Resolution | 24.580 - 1.100 |
| Rwork | 0.182 |
| R-free | 0.19650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zo8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.433 |
| Data reduction software | XDS (0.86) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.20) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.580 | 1.139 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rpim | 0.030 | 0.900 |
| Number of reflections | 227592 | 6262 |
| <I/σ(I)> | 12.99 | 0.65 |
| Completeness [%] | 78.3 | 22.6 |
| Redundancy | 4.3 | 3.1 |
| CC(1/2) | 0.999 | 0.244 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES pH 6.5 25% PEG 1500 |
