7R3W
Crystal structure of the albicidin resistance protein STM3175 from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 120.650, 244.920, 139.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.420 - 3.600 |
| R-factor | 0.2702 |
| Rwork | 0.268 |
| R-free | 0.31450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | The search model was computed by the Robetta Server |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.685 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.820 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmeas | 0.233 | 1.969 |
| Number of reflections | 24093 | 3804 |
| <I/σ(I)> | 8.17 | 0.98 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 5.6 | 5.9 |
| CC(1/2) | 0.996 | 0.460 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M magnesium formate |
