7R2Y
Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in complex with ATP resulting from short ATP soak, conflicting T-loop and C-loop with partial occupancy
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 63.833, 63.833, 81.231 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.730 - 2.150 |
| R-factor | 0.1817 |
| Rwork | 0.180 |
| R-free | 0.21220 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5o3p |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.957 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.730 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.044 | 1.395 |
| Number of reflections | 20143 | 3177 |
| <I/σ(I)> | 27 | |
| Completeness [%] | 99.6 | |
| Redundancy | 10.2 | |
| CC(1/2) | 1.000 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2M SODIUM ACETATE, 0.1M HEPES PH 7.5, 20% (W/V) PEG 3000 |
