7R2E

Structure of human Senp7 with SUMO2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	36.413, 100.730, 93.037
Unit cell angles	90.00, 90.28, 90.00

Refinement procedure

Resolution	50.360 - 1.740
R-factor	0.1863
Rwork	0.184
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eay
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.030	1.900
High resolution limit [Å]	1.730	1.730
Number of reflections	51898	2595
<I/σ(I)>	10.7
Completeness [%]	92.2
Redundancy	3.3
CC(1/2)	0.999	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Sodium HEPES 7.0, 15 % w/v PEG 20,000